SoRa Objective Lenses

Owned by Ki Hng
Last updated: May 01, 2024 by Andrew Vaughan

Currently on the microscope (updated February 2023)

ObjectiveMagnificationImmersion MediumNumerical ApertureCoverslip correction (mm)Working Distance (mm)DIC PrismCondenser Prism
CFI Plan Apochromat Lambda D 20x20xAir0.80.170.8N/AN/A
CFI Plan Apo Lambda S 40XC Sil40xSilicone1.250.13-0.210.340x IN2
CFI Apo Lambda S 40XC WI40xWater1.250.15-0.190.2-0.1640x IN2
CFI SR Plan Apo IR 60XC WI60xWater1.270.15-0.190.18-0.1660x IV-RNR
CFI Apo TIRF 60XC Oil60xOil1.490.13-0.190.16-0.07N/AN/A
CFI SR HP Apo TIRF 100XC Oil100xOil1.490.13-0.190.1-0.16100x I-RNR


Available to request (email lmcb-lm-help@ucl.ac.uk)

ObjectiveMagnificationImmersion MediumNumerical ApertureCoverslip Correction (mm)Working Distance (mm)DIC PrismCondenser Prism
CFI Plan Apochromat Lambda D 4x4xAir0.20-0.1720N/AN/A
CFI Plan Apochromat Lambda D 10x10xAir0.450.17

4

10xN1
CFI Apo LWD Lambda S 20XC WI20xWater0.950.11-0.23

0.99-0.9

20x IIIN2
CFI Apo LWD Lambda S 40XC WI40xWater1.150.15-0.19

0.61-0.59

40x IN2
CFI Plan Apo Lambda S 25XC Sil25xSilicone1.050.11-0.23

0.55

25x IIN2
CFI SR HP Plan Apo Lambda S 100XC Sil100xSilicone1.350.15-0.190.31-0.28100x I-RNR
CFI Plan Fluor 40x Oil40xOil1.30.170.2440x IN2
CFI Plan Apochromat Lambda D 60x Oil60xOil1.420.170.1560x IIN2

Filter sets in the CSU-W1 SoRa scan head

  1. DM Wheel (primary dichroic mirror)
  2. Light Path Changer (secondary dichroic mirror)
  3. EM1 Wheel (emission filter wheel for Camera 1)
  4. EM2 Wheel (emission filter wheel for Camera 2)

DM Wheel

Position


1DAPI/FITC/TRITC/Cy5 Quad Band DMMXR00753 - LED - DA/FI/TR/Cy5-B (DAPI/FITC/TRITC/Cy5 Full Multiband Quad) (FF409/493/573/652 - Di02) (Di)Confocal using 405, 488, 561 and 638 nm lasers
2CFP/YFP/mCherry Triple Band DMMXR00723 - LED - CFP/YFP/mCherry-A (CFP/YFP/mCherry - Full Multiband Triple) MXR00537 445/514/594 DM for CSU-W1Confocal using 445, 515 and 561 nm lasers
3350-700 MirrorMXRS1176 Semrock Mirror for CSU-W1For wide-field mode

Light Path Changer

Position

1DM A561LPDirects light of wavelength > 561 nm to Camera 1 for simultaneous Green/Red imaging
2DM A514LPDirects light of wavelength > 514 nm to Camera 1 for simulatneous CFP/YFP imaging
3VIS-AAll light goes to Camera 1 for single camera imaging

EM1 Wheel

Position


1
MXR00753 - LED - DA/FI/TR/Cy5-B (DAPI/FITC/TRITC/Cy5 - Full Multiband Quad) (FF01 - 432/515/595/730) (Em)
2447/60MXRS0705 447/60 BrightLine single-band DAPIDAPI
3472/30MXRS0705 472/30 BrightLine single-band CFPCFP
4525/50MXRS0755 525/50 BrightLine single-bandGreen
5542/27MXRS0400 Brightline single-band bandpass YFP emission filterYFP
6600/52MXRS0503 BrightLine single-band bandpass FF01-600/52-25Red
7641/75MXRS0741 BrightLine single-band bandpass Texas RedTexas Red
8708/75MXRS0612 708/75 BrightLine single-band Cy5Cy5
9809/89

10Full

EM2 Wheel

Position


1
LED - DA/FI/TR/Cy5-B (DAPI/FITC/TRITC/Cy5 - Full Multiband Quad) (FF01 - 432/515/595/730) (Em)
2432/36

3447/60MXRS0705 447/60 BrightLine single-band DAPIDAPI
4472/30MXRS0705 472/30 BrightLine single-band CFPCFP
5515/30

6525/50MXRS0755 525/50 BrightLine single-bandGreen
7542/27MXRS0400 Brightline single-band bandpass YFP emission filterYFP
8Empty

9Empty

10Full