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- Click on the LAS X Navigator icon.
- Click on the Z-Stack button.
- Make sure Same Stack Size for all Regions is off.
- Go to the first position and set the parameters for the Z-stack. Define the beginning (Begin) and end (End) of the z-stack, determine the number (Nr. of Steps) and size (Z-Step Size) of the steps, or use System Optimised.
- Go to the centre of the z-stack or to another plane of focus if the plane of focus is not in the middle of the stack.
- Click Mark to define the first scan region.
- Move to the next scan region, set up the z-stack parameters and mark the point.
- Repeat for all other points.
- In the Task List, the Z position and number of sections is shown for the images (i.e. marked points) as you hover the mouse over them, which will indicate that the stacks for each are different.
