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The SPE3 is a semi-automated, inverted Leica TCS SPE confocal microscope with an automated stage and z galvanometer insert. It will take slides, dishes and plates, but please not that all culture-style vessels must have glass bottoms of standard #1.5 coverslip thickness (170 µm). The confocal scanning system has the following lasers:
Lasers and Detectors
- 405 nm (violet, for blue dyes like DAPI)
- 488 nm (blue, for green dyes like GFP)
- 561 nm (lime green, for red dyes like mCherry)
- 635 nm (red, for far red dyes like Cy5)
It has a quad dichroic mirror meaning a rapid switch between channels.
There is only one photomultiplier tube (PMT) so all multi-channel acquisition is done sequentially
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