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The SPE3 is a semi-automated, inverted Leica TCS SPE confocal microscope with an automated stage and z galvanometer insert. It will take slides, dishes and plates, but please not that all culture-style vessels must have glass bottoms of standard #1.5 coverslip thickness (170 µm).
There is only one photomultiplier tube (PMT) so all multi-channel acquisition is done sequentially
A galvanometer driven stage insert is used to change the focus when optically sectioning samples. The speed of this device means that XZY scans are possible in addition to the conventional XYZ scan.
The ACS objective lenses, which have been designed to correct for colour registration errors caused by chromatic aberration, do not have a flat field, so best results are achieved at zooms ≥1.6.
There is also the option on this machine to carry out small screens thanks to a matrix screener software
The confocal scanning system has the following lasers:
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