Page Comparison

h2. Leica TCS SPE confocal microscope (SPE3)


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*Location:* *MRC Building, Room 2.16*

{float:right}!SPE3.jpg|border=1!{float}

The SPE3 is a second2012 generationmodel Leica TCS SPE confocal microscope mounted on a semi-automated, inverted Leica DMI4000 stand. The stand is capable of fluorescence, bright-field and DIC techniques and is also fitted with a motorised stage for multi-point (Mark & Find) and stitching (Tile Scan) acquisitions. The confocal system has four lasers and a single photomultiplier tube (PMT) detector soao all multichannel acquisition must be done sequentially. The microscope stand has a manual focus drive so optical sectioning is achieved using the Z Galvo insert integrated into the stage.The software has Tile Scan and Mark  & Find modes, which use the  microscope's motorised stage to revisit  saved positions or build up a  'mosaic' of images of adjacent areas.

The ACS objective lenses have been designed to correct for  colour registration errors between the violet and visible lasers. They do not have a  flat field so Leica recommend that the zoom is set to ≥1.6. There is also the option on this machine to carry out small screens thanks to a matrix screener software.

| *Contents* |
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h3. Access & Usage
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* [Training|LMCBLMic:LMCB Microscope Training]
* [Confocal Booking System (Faces Scheduling System)|http://faces.ccrc.uga.edu/]
* [Microscope Booking Rules|LMCBLMic:Microscope Booking]

h3. Technical Specifications
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* [Microscope Stand Information|SPE3 Microscope Information]

h2.

h3. User Guides
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* [Start-up and Shut-down Procedure|^SPE3_StartUp_Shut Down.pdf]
* [Transmitted Light DIC on SPE3|^TransmittedLightImagesontheSPE.pdf]
* [Confocal Resolution Calculator|http://lightmicroscopy.lmcb.ucl.ac.uk/Information/LSM_Res.html]