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h2. Leica TCS SPE confocal microscope (SPE3)


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*Location:* *MRC Building, Room 2.16*

{float:right}!SPE3.jpg|border=1!{float}

The SPE3 is a 2012 model Leica TCS SPE confocal microscope mounted on a semi-automated, inverted Leica DMI4000 stand. The stand is capable of fluorescence, bright-field and DIC techniques and is fitted with a motorised stage. The confocal system has four lasers and a single photomultiplier tube (PMT) detector so multichannel acquisition must be done sequentially. The microscope stand has a manual focus drive so optical sectioning is achieved using the Z Galvo insert integrated into the stage.The software has Tile Scan and Mark  & Find modes, which use the  microscope's motorised stage to revisit  saved positions or build up a  'mosaic' of images of adjacent areas.


| *Contents* |
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h3. Access & Usage
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* [Training|LMCBLMic:LMCB Microscope Training]
* [Confocal Booking System (Faces Scheduling System)|http://faces.ccrc.uga.edu/]
* [Microscope Booking Rules|LMCBLMic:Microscope Booking]

h3. Technical Specifications
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* [Microscope Components (Objective lenses, filter sets, lasers, etc|LMCBLMic:SPE3 Microscope Information])


h3. User Guides
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* [Start-up and Shut-down Procedure|^SPE3_StartUp_Shut Down.pdf]
* [Using the buttons on the DMI4000 microscope for fluorescence, bright-field or DIC|SPE3 DMI4000 buttons]
* [Transmitted Light DIC on SPE3|^TransmittedLightImagesontheSPE.pdf]
* [Confocal Resolution Calculator|http://lightmicroscopy.lmcb.ucl.ac.uk/Information/LSM_Res.html]