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h2. Leica TCS SPE confocal microscope (SPE3) ---- *Location:* *MRC Building, Room 2.16* {float:right}!SPE3.jpg|border=1!{float} The SPE3 is a 2012 model Leica TCS SPE confocal microscope mounted on a semi-automated, inverted Leica DMI4000 stand. The stand is capable of fluorescence, bright-field and DIC techniques and is fitted with a motorised stage. The confocal system has four lasers and a single photomultiplier tube (PMT) detector so multichannel acquisition must be done sequentially. The microscope stand has a manual focus drive so optical sectioning is achieved using the Z Galvo insert integrated into the stage.The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas. | *Contents* | | {toc:type=list|minLevel=3|maxLevel=3} | h3. Access & Usage ---- * [Training|LMCBLMic:LMCB Microscope Training] * [Confocal Booking System (Faces Scheduling System)|http://faces.ccrc.uga.edu/] * [Microscope Booking Rules|LMCBLMic:Microscope Booking] h3. Technical Specifications ---- * [Microscope Components (Objective lenses, filter sets, lasers, etc|LMCBLMic:SPE3 Microscope Information]) h3. User Guides ---- * [Start-up and Shut-down Procedure|^SPE3_StartUp_Shut Down.pdf] * [Using the buttons and other controls on the DMI4000 microscope for fluorescence, bright-field or DIC|SPE3 DMI4000 buttonscontrols] * [Transmitted Light DIC on SPE3|^TransmittedLightImagesontheSPE.pdf] * [Confocal Resolution Calculator|http://lightmicroscopy.lmcb.ucl.ac.uk/Information/LSM_Res.html] |
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