DMI4000
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microscope
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The SPE confocal system is built around attached to a motorised DM4000 microscope stand. The fluorescence filter turret and objective lens nosepiece nose-piece are manually rotated . There is currently no internal focus motor; but the positions are encoded so the software knows which objective lens is in place. The microscope stand focus in manual so confocal sectioning is achieved via a galvanometer driven stage insert (Z Galvo). Eyepiece or confocal light path selection is automated. DIC is available on this microscope.
Objective Lenses on the Leica DM4000 Microscope
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Turret Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
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1 | A | BP340-380 | RKP400 | LP425 | Hoechst/DAPI |
2 | I3 | BP450-490 | RKP510 | LP515 | FITC/GFP |
3 | N2.1 | BP515-560 | RKP580 | LP590 | Texas Red |
4 | BF | N/A | N/A | N/A | Transmitted Light |
5 | N/A | N/A | N/A | N/A | Empty |
6 | Pol | N/A | N/A | N/A | Polariser for DIC and polarised light microscopy |
Positions 4 to 6 are empty and not used in this standfor fluorescence microscopy.