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Objective Lenses
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Leica ACS objective lenses have been designed specifically for SPE confocal microscopes with 405 nm lasers to correct for colour registration errors between the violet and visible lines. They do not have a flat field so Leica recommends that the confocal zoom is set to ≥1.6.
Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction | DIC Prism |
|---|---|---|---|---|---|
10X | Air | ACS APO | 0.3 | None | C |
20X | Multi Imm | ACS APO | 0.6 | 0 - 0.17 | |
40X | Oil | ACS APO | 1.15 | 0.17 | E |
63X | Oil | ACS APO | 1.3 | 0.17 | E |
Fluorescence Filter Sets
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Turret Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
|---|---|---|---|---|---|
1 | A | BP340-380 | RKP400 | LP425 | Hoechst/DAPI |
2 | I3 | BP450-490 | RKP510 | LP515 | FITC/GFP |
3 | N2.1 | BP515-560 | RKP580 | LP590 | Texas Red |
4 | BF | N/A | N/A | N/A | Transmitted Light |
5 | N/A | N/A | N/A | N/A | Empty |
6 | Pol | N/A | N/A | N/A | Polariser for DIC and polarised light microscopy |
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