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Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction | DIC Prism |
|---|---|---|---|---|---|
10X | Air | ACS APO | 0.3 | None | C |
20X | Multi Imm | ACS APO | 0.6 | 0 - 0.17 | |
40X | Oil | ACS APO | 1.15 | 0.17 | E |
63X | Oil | ACS APO | 1.3 | 0.17 | E |
Fluorescence Filter Sets (for observation down the eyepieces)
Turret Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
|---|---|---|---|---|---|
1 | A | BP340-380 | RKP400 | LP425 | Hoechst/DAPI |
2 | I3 | BP450-490 | RKP510 | LP515 | FITC/GFP |
3 | N2.1 | BP515-560 | RKP580 | LP590 | Texas Red |
4 | BF | N/A | N/A | N/A | Transmitted Light |
5 | N/A | N/A | N/A | N/A | Empty |
6 | Pol | N/A | N/A | N/A | Polariser for DIC and polarised light microscopy |
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