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Fluorescence filter sets (for observation down the eyepieces)
These fluorescence filters are only used when you're focusing on your specimen down the eyepieces. The filter turret is automatically rotated to the empty Position 4 (BF) when a confocal scan is initiated.
Turret Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
|---|---|---|---|---|---|
1 | A | BP340-380 | RKP400 | LP425 | Hoechst/DAPI |
2 | I3 | BP450-490 | RKP510 | LP515 | FITC/GFP |
3 | N2.1 | BP515-560 | RKP580 | LP590 | Texas Red |
4 | BF | N/A | N/A | N/A | Transmitted Light |
5 | N/A | N/A | N/A | N/A | Empty |
6 | Pol | N/A | N/A | N/A | Polariser for DIC and polarised light microscopy |
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