Troubleshooting
If you link to this troubleshooting page from outside the wiki then please either scroll down or use the following Table of Contents to find the right section
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Leica Confocals
What to do if the LASAF software says you don't have a valid licence when you try to start the confocal
- Quit the software
- Unplug the licence dongle from the back of the computer and plug it back in again
- Restart the software
If you shut the system down - or just restart the computer - that should also work
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If you ever need to use the Focus Stop for any reason please disengage it once you have finished. Also please be careful not to accidentally engage the Focus Stop when you are adjusting the focus knob.
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If you are using the STED as a confocal you may from time-to-time notice that the controls for scan field rotation are not visible in the software or on the control panel. This is because the microscope has been used for STED and scan field rotation is disabled so the illumination and depletion lasers remain aligned. To regain control of scan field rotation you need to quit and reopen the software and turn off the STED switch when you see it on the splash screen.
Solent Scientific Chambers
Volocity Time-lapse Microscopes
GFP2: What to do if all the devices are on but when you open Volocity there are no controls for the microscope, filters, shutters or XY stage
This is a software bug where the volocity.exe process does not close when you quit Volocity. This means that if you reopen Volocity the earlier process will still be talking to the microscope and Ludl controller and will deny you access.
Workaround solution:
- Quit Volocity
- Press CTRL ALT DEL to open the Task Manager
- Click on the Processes tab
- Re-order the processes alphabetically by clicking Image Name at the top of the column and scroll down to find volocity.exe
- There may be several instances of volocity.exe - select and end them all
- Close Task Manager and re-open Volocity