| Objective Lenses | ||||||
|---|---|---|---|---|---|---|
Magnification | Immersion Medium | DesignationType | Numerical Aperture | Coverslip Correction | Working DistanceNA | WD (mm) |
10X | Air | Plan Apo | 0.450.17 | 4 | ||
20X | Air | CFI Plan Apochromat VC | 0.750.17 | 1 | ||
40X | Air | CFI Plan Fluor | 0.75 | 0.170.72 | ||
60X | Oil | CFI Plan Apochromat | 1.40.17 | 0.13 | ||
100X | Oil | CFI Plan Apochromat VC | 1.40.17 | 0.13 | ||
60X WI | Water | CFI Plan Apochromat | 1.2 | 0.17 | 0.27 | |
...
27 | ||||
| All lenses are corrected for #1.5 coverslips (0.17 micron thick). NA = Numerical Aperture, WD = Working Distance | ||||
There is a choice of three immersion oils:
Oil | Refractive Index | For use with... | Kept in... |
|---|---|---|---|
Cargille Type HF | 1.518 (at 23ºC) | Oil lenses at room temperature | Tray outside environmental chamber |
Cargille Type 37 | 1.518 (at 37°C) | Oil lenses at 37ºC | Tray inside environmental chamber |
Zeiss Immersol W | 1.334 (at 23ºC) | Water immersion lens only | Tray inside environmental chamber |
...