Ultraview Vox Objectives

Objective Lenses
Magnification	Immersion	Type	NA	WD (mm)
10X	Air	Plan Apo	0.45	4
20X	Air	CFI Plan Apochromat VC	0.75	1
40X	Air	CFI Plan Fluor	0.75	0.72
60X	Oil	CFI Plan Apochromat	1.4	0.13
100X	Oil	CFI Plan Apochromat VC	1.4	0.13
60X WI	Water	CFI Plan Apochromat	1.2	0.27
All lenses are corrected for #1.5 coverslips (0.17 micron thick). NA = Numerical Aperture, WD = Working Distance

There is a choice of three immersion oils:

Oil	Refractive Index	For use with...	Kept in...
Cargille Type HF	1.518 (at 23ºC)	Oil lenses at room temperature	Tray outside environmental chamber
Cargille Type 37	1.518 (at 37°C)	Oil lenses at 37ºC	Tray inside environmental chamber
Zeiss Immersol W	1.334 (at 23ºC)	Water immersion lens only	Tray inside environmental chamber

Note that Cargille Type 37 is optimal for use at 37ºC but introduces spherical aberrations when imaging beyond about 10 μm in aqueous media because it is not refractive indexed matched to water. Zeiss Immersol W in combination with the 60x WI lens might be a better choice in this case but its RI is corrected for use at room temperature and won't be correct at 37 degrees.