It relies on a motorised dichroic mirror to switch between illumination conditions, which is too slow for very rapid processes (< 1 sec).
Both machines have four lasers and a single photomultiplier tube (PMT) detector, so simultaneous acquisition of multiple channels is not possible. All multi-channel acquisition is done sequentially. A galvanometer driven stage insert is used to change the focus when optically sectioning samples. The speed of this device means that XZY scans are possible in addition to the conventional XYZ scan and single colour 3D time series acquisitions can be quite fast. The ACS objective lenses, which have been designed to correct for colour registration errors caused by chromatic aberration, do not have a flat field, so best results are achieved at zooms ≥1.6. |