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Leica Microsystems Stellaris 8 DIVE

 Technical information and user guides

Location: Medawar Building. Room 2.19B.

The DIVE (Deep In-Vivo Explorer) has the following features:

  • A fixed-stage upright DM6 microscope stand
  • The choice of standard objective lenses and special long-working distance dipping lenses for imaging deep into live and cleared specimens
  • A dual-beam Coherent Discovery multiphoton laser with a tunable laser line and a fixed 1040 nm laser line
  • A range of visible lasers for confocal imaging
  • Spectrally tuneable imaging with non-descanned detectors using the 4Tune system.

Technical Specifications

Lasers

The DIVE has the following visible solid state lasers for confocal microscopy.

WavelengthColourExample dyes
405
DAPI, Hoechst
448
CFP
488
GFP, Alexa 488
514
YFP
561
mCherry, Alexa 568
638
Cy5, Alexa 647

The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm. The fixed and tuneable lines can be used simultaneously.

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