DIVE lasers
The DIVE has the following visible solid state lasers for confocal microscopy.
Wavelength | Colour | Example dyes |
|---|---|---|
405 |
| DAPI, Hoechst |
448 |
| CFP |
488 |
| GFP, Alexa 488 |
514 |
| YFP |
561 |
| mCherry, Alexa 568 |
638 |
| Cy5, Alexa 647 |
The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm. The fixed and tuneable lines can be used simultaneously.