GENERAL INFORMATION

The Zeiss ELYRA PS.1 at the UCL MRC Laboratory of Molecular Cell Biology.

GENERAL INFORMATION

The Zeiss ELYRA PS.1 is capable of both Structured Illumination Microscopy (SIM) and Photo-Activation Localisation Microscopy (PALM). In addition it can be configured for dSTORM, standard TIRF and wide-field microscopy applications. The microscope stage is enclosed in a blacked-out chamber for laser safety reasons and to maintain thermal stability. Super-resolution imaging is recommended and optimal at ambient temperature, but stage-top incubation equipment for live cell imaging applications is available. Multi-colour super-resolution imaging is also possible. The microscope is equipped with four laser lines 405, 488, 561 and 642 nm. The microscope is located in room G-09.

CONTENTS

QUICK START INSTRUCTIONS - Hardware & Software

Zeiss ELYRA PS.1 - Quick Shutdown Instructions

GETTING STARTED – Putting a Specimen on and Locating it Using the Ocular

GETTING STARTED – SIM Imaging & Image Processing

GETTING STARTED – PALM & 3D PALM Imaging & Image Processing

Zeiss ELYRA PS.1 - Technical Specifications

LINKS

Background information on super-resolution techniques

Introduction to SIM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/supersim.html

Interactive SIM tutorial: http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/hrsim/indexflash.html

Introduction to PALM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/palm/introduction.html