GENERAL INFORMATION
The Zeiss ELYRA PS.1 at the UCL MRC Laboratory of Molecular Cell Biology.
GENERAL INFORMATION
The Zeiss ELYRA PS.1 is capable of both Structured Illumination Microscopy (SIM) and Photo-Activation Localisation Microscopy (PALM). In addition it can be configured for dSTORM, standard TIRF and wide-field microscopy applications. The microscope stage is enclosed in a blacked-out chamber for laser safety reasons and to maintain thermal stability. Super-resolution imaging is recommended and optimal at ambient temperature, but stage-top incubation equipment for live cell imaging applications is available. Multi-colour super-resolution imaging is also possible. The microscope is equipped with four laser lines 405, 488, 561 and 642 nm. The microscope is located in room G-09.
CONTENTS
QUICK START INSTRUCTIONS - Hardware & Software
Zeiss ELYRA PS.1 - Quick Shutdown Instructions
GETTING STARTED – Putting a Specimen on and Locating it Using the Ocular
GETTING STARTED – SIM Imaging & Image Processing
GETTING STARTED – PALM & 3D PALM Imaging & Image Processing
Zeiss ELYRA PS.1 - Technical Specifications
LINKS
Background information on super-resolution techniques
Introduction to SIM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/supersim.html
Interactive SIM tutorial: http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/hrsim/indexflash.html
Introduction to PALM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/palm/introduction.html
Practical aspects of PALM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/palm/practicalaspects.html
Interactive PALM tutorial: http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/palmbasics/indexflash.html
Sample preparation
Zeiss ELYRA Sample Preparation Quick Guide 2013.pdf
Additional information on the Zeiss ELYRA PS.1
Zeiss ELYRA - Enter the World of Super-Resolution Brochure 2012.pdf
Useful image analysis tools
SIMCheck to test the quality of your SIM images: http://www.micron.ox.ac.uk/software/SIMCheck.php
Super-Resolution Facility (SurF) access application form
http://www.ucl.ac.uk/lmcb/Super-Resolution-access-form