| Objective Lenses | |||||
|---|---|---|---|---|---|
| Magnification | Immersion | Type | Phase Contrast | NA | WD (mm) |
| 10x | Air | Plan Fluor | Ph1 | 0.3 | 15.2 |
| 20x | Air | Plan Fluor | Ph1 | 0.5 | 2.1 |
| 40x | Air | Plan Fluor | Ph2 | 0.75 | 0.66 |
| 60x | Oil | Plan Apo λ | Ph3 | 1.4 | 0.13 |
| 40x | Air | Plan Fluor ELWD | Ph2 | 0.6 | 3.7 |
| 20x | Air | Plan Fluor ELWD | Ph1 | 0.45 | 7.4 |
Lenses are corrected for #1.5 coverslips (0.17 micron thick) except ELWD (extra long working distance) NA = Numerical Aperture, WD = Working Distance | |||||
There is a choice of two immersion oils:
Oil | Refractive Index | For use with... | Kept in... |
|---|---|---|---|
Cargille Type HF | 1.518 (at 23ºC) | Oil lenses at room temperature | Tray outside environmental chamber |
Cargille Type 37 | 1.518 (at 37°C) | Oil lenses at 37ºC | Tray inside environmental chamber |
Note that Cargille Type 37 is optimal for use at 37ºC but introduces spherical aberrations when imaging beyond about 10 μm in aqueous media because it is not refractive indexed matched to water.