The resolution of light microscopy has traditionally been limited to approximately half the wavelength of light, which means anything smaller than about 250 nm cannot be resolved. Super-resolution microscopy techniques extend light microscopy beyond that barrier.
About Super-resolution Microscopy…
The Super-resolution Facility at the LMCB (SuRF) offers a suite of complementary super-resolution microscope technologies.
Super-resolution Microscopes
- Carl Zeiss Elyra PS.1 - Structured Illumination Microscopy (SIM) and Single molecule Localisation Microscopy (SMLM)
- Leica SP8 STED 3X - Stimulated Emission Depletion (STED) microscopy
- Nikon SoRa - Image Scanning Microscopy (ISM)
- ONI Nanoimager - SMLM, single molecule tracking, smFRET
- 3i Lattice LightSheet – Lattice light sheet microscopy
Using the facility
The achievable resolution and suitability of each technique depends on the specimen and the experimental requirements. If you are interested in using the facility, then please fill in this short web form and the facility staff will arrange a meeting to discuss your requirements.
The charge for using the facility is currently £33.28 per hour. SuRF is a Multi-User Facility (MUF), which cannot be selected in the WorkTribe Research management System, so for grant applications you will need a quotation from the facility manager. Please email Andrew Vaughan for more information or a quote.