Leica Microsystems Stellaris 8 DIVE
Location: Medawar Building. Room 2.19B.
The DIVE (Deep In-Vivo Explorer) has the following features:
- A fixed-stage upright DM6 microscope stand
- The choice of standard objective lenses and special long-working distance dipping lenses for imaging deep into live and cleared specimens
- A dual-beam Coherent Discovery multiphoton laser with a tunable laser line and a fixed 1040 nm laser line
- A range of visible lasers for confocal imaging
- Spectrally tuneable imaging with non-descanned detectors using the 4Tune system.
Technical Specifications
Lasers
The DIVE has the following visible solid state lasers for confocal microscopy.
Wavelength | Colour | Example dyes |
---|---|---|
405 | DAPI, Hoechst | |
448 | CFP | |
488 | GFP, Alexa 488 | |
514 | YFP | |
561 | mCherry, Alexa 568 | |
638 | Cy5, Alexa 647 |
The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm. The fixed and tuneable lines can be used simultaneously.