Leica TCS SP5 Confocal Microscope
Location: MRC Building, first floor, room 1.20B
Section |
---|
Column |
---|
| The SP5 is a flexible, fully-featured laser scanning confocal microscope suitable for imaging fixed and live samples. It has an inverted DMI6000B microscope stand capable of fluorescence, bright-field and DIC techniques. The SP5 is a tandem scanner system, which means that it has both a conventional scanner and a fast (8000Hz) resonant scanner for high speed imaging. It is fitted with 5 lasers, producing 8 potential excitation lines, and has 4 PMT detectors and one transmitted light detector. Optical sectioning of samples is achieved using the microscope's integrated focus drive. The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas. FRAP, FRET AB (Acceptor Photobleaching), FRET SE (Sensitised Emission) and Live Data Mode modules are also included with the software. A Pe-Con stage-top incubation system with heat and CO2 control is available for live samples. |
|
Expand |
---|
title | Technical Specifications |
---|
|
Microscope Information |
...
Confocal Resolution Calculator
Start-up Procedure
Shut-down Procedure
Image Acquisition
Using the Hybrid Detectors
How to insert the sample holder
Image Orientation
Orientation of Stage Movement
Using Tile Scan
Direction of Tile Scanning when seen on the display
Correcting fluorescence channel mis-registration when using DIC
Correcting chromatic shift using Volocity
Making symmetric stacks in Mark & Find mode
Making asymmetric stacks in Mark & Find mode using Define Asymmetry
...