SP5 Microscope Information
Leica DMI6000 Microscope
Objective Lenses on the Leica DMI6000 Microscope
Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction (mm) | Working Distance (mm) |
|---|---|---|---|---|---|
10X | Air | HC PL FLUOTAR | 0.3 | None | 11 |
20X | Multi | HC PL APO | 0.7 | 0 - 0.17 | 0.26 |
40X | Oil | HCX PL APO | 0.75 - 1.25 | 0.17 | 0.1 |
63X | Oil | HCX PL APO | 0.6 - 1.4 | 0.17 | 0.1 |
*63X | Glycerol 21°C | HCX PL APO | 0.75 - 1.25 | 0.14 - 0.18 | 0.28 |
*40X | Air | HCX PL FLUOTAR | 0.75 | 0.17 | 0.4 |
* These objective lenses are available but aren't routinely fitted to the microscope
Fluorescence Filter Sets on the Leica DMI6000 Microscope
Turret Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
|---|---|---|---|---|---|
1 | I3 | BP450-490 | RKP510 | LP515 | FITC/GFP |
2 | N2.1 | BP515-560 | RKP580 | LP590 | Texas Red |
3 | Pol | N/A | N/A | N/A | Polariser for DIC and polarised light microscopy |
4 | BF | N/A | N/A | N/A | Empty for transmitted light and confocal scanning |
5 | A | BP340-380 | RKP400 | LP425 | Hoechst/DAPI |
6 | CFP | BP 436/20 | 455 | BP 480/40 | CFP |
The filter positions above control what the user sees down the microscope while preparing his/her sample and not what light is acquired during a confocal scan.
Buttons 3 and 4 are not active; the transmitted light illumination mode is controlled by the TL/IL and ChTL buttons.