SP5 Microscope Information

Leica DMI6000 Microscope

Objective Lenses on the Leica DMI6000 Microscope

Magnification	Immersion Medium	Designation	Numerical Aperture	Coverslip Correction (mm)	Working Distance (mm)
10X	Air	HC PL FLUOTAR	0.3	None	11
20X	Multi	HC PL APO	0.7	0 - 0.17	0.26
40X	Oil	HCX PL APO	0.75 - 1.25	0.17	0.1
63X	Oil	HCX PL APO	0.6 - 1.4	0.17	0.1
*63X	Glycerol 21°C	HCX PL APO	0.75 - 1.25	0.14 - 0.18	0.28
*40X	Air	HCX PL FLUOTAR	0.75	0.17	0.4

* These objective lenses are available but aren't routinely fitted to the microscope

Fluorescence Filter Sets on the Leica DMI6000 Microscope

Turret Position	Filter Set	Excitation Filter	Dichroic Mirror	Emission Filter	Example Fluorophores
1	I3	BP450-490	RKP510	LP515	FITC/GFP
2	N2.1	BP515-560	RKP580	LP590	Texas Red
3	Pol	N/A	N/A	N/A	Polariser for DIC and polarised light microscopy
4	BF	N/A	N/A	N/A	Empty for transmitted light and confocal scanning
5	A	BP340-380	RKP400	LP425	Hoechst/DAPI
6	CFP	BP 436/20	455	BP 480/40	CFP

The filter positions above control what the user sees down the microscope while preparing his/her sample and not what light is acquired during a confocal scan.

Buttons 3 and 4 are not active; the transmitted light illumination mode is controlled by the TL/IL and ChTL buttons.