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Leica
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TCS
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SP5
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Confocal
...
Microscope
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Location:
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MRC
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Building,
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first
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floor,
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room
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1.20B
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The
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SP5
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is
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a
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flexible,
...
fully-featured
...
laser
...
scanning
...
confocal
...
microscope
...
suitable
...
for
...
imaging
...
fixed
...
and
...
live
...
samples.
...
It
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has
...
an
...
inverted
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DMI6000B
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microscope
...
stand
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capable
...
of
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fluorescence,
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bright-field
...
and
...
DIC
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techniques.
...
The
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SP5
...
is
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a
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tandem
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scanner
...
system,
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which
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means
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that
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it
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has
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both
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a
...
conventional
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scanner
...
and
...
a
...
fast
...
(8000Hz)
...
resonant
...
scanner
...
for
...
high
...
speed
...
imaging.
...
It
...
is
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fitted
...
with
...
5
...
lasers,
...
producing
...
8
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potential
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excitation
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lines,
...
and
...
has
...
4
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PMT
...
detectors
...
and
...
one
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transmitted
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light
...
detector.
...
Optical
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sectioning
...
of
...
samples
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is
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achieved
...
using
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the
...
microscope's
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integrated
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focus
...
drive.
...
The
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software
...
has
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Tile
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Scan
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and
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Mark
...
&
...
Find
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modes,
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which
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use
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the
...
microscope's
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motorised
...
stage
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to
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revisit
...
saved
...
positions
...
or
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build
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up
...
a
...
'mosaic'
...
of
...
images
...
of
...
adjacent
...
areas.
...
FRAP,
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FRET
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AB
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(Acceptor
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Photobleaching),
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FRET
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SE
...
(Sensitised
...
Emission)
...
and
...
Live
...
Data
...
Mode
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modules
...
are
...
also
...
included
...
with
...
the
...
software.
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A
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Pe-Con
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stage-top
...
incubation
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system
...
with
...
heat
...
and
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CO2
...
control
...
is
...
available
...
for
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live
...
samples.
...
Contents | ||||||
|
Access & Usage
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Technical Specifications
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User Guides
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- Confocal Resolution Calculator
- Start-up Procedure
- Shut-down Procedure
- Image Acquisition
- Using the Hybrid Detectors
- How to insert the sample holder
- Image Orientation
- Orientation of Stage Movement
- Using Tile Scan
- Direction of Tile Scanning when seen on the display
- Correcting fluorescence channel mis-registration when using DIC