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Leica
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TCS
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SP5
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Confocal
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Microscope
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Location:
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MRC
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Building,
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first
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floor,
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room
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1.20B
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The
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SP5
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is
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a
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flexible,
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fully-featured
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laser
...
scanning
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confocal
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microscope
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suitable
...
for
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imaging
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fixed
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and
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live
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samples.
...
It
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has
...
an
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inverted
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DMI6000B
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microscope
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stand
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capable
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of
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fluorescence,
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bright-field
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and
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DIC
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techniques.
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The
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SP5
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is
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a
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tandem
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scanner
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system,
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which
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means
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that
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it
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has
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both
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a
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conventional
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scanner
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and
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a
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fast
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(8000Hz)
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resonant
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scanner
...
for
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high
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speed
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imaging.
...
It
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is
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fitted
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with
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5
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lasers,
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producing
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8
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potential
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excitation
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lines,
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and
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has
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4
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PMT
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detectors
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and
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one
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transmitted
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light
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detector.
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Optical
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sectioning
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of
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samples
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is
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achieved
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using
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the
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microscope's
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integrated
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focus
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drive.
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The
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software
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has
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Tile
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Scan
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and
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Mark
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&
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Find
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modes,
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which
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use
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the
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microscope's
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motorised
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stage
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to
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revisit
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saved
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positions
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or
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build
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up
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a
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'mosaic'
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of
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images
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of
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adjacent
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areas.
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FRAP,
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FRET
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AB
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(Acceptor
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Photobleaching),
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FRET
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SE
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(Sensitised
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Emission)
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and
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Live
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Data
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Mode
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modules
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are
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also
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included
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with
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the
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software.
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A
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Pe-Con
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stage-top
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incubation
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system
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with
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heat
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and
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CO2
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control
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is
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available
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for
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live
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samples.
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Contents | ||||||
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Access & Usage
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Technical Specifications
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User Guides
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- Confocal Resolution Calculator
- Start-up Procedure
- Shut-down Procedure
- Image Acquisition
- Using the Hybrid Detectors
- How to insert the sample holder
- Image Orientation
- Orientation of Stage Movement
- Using Tile Scan
- Direction of Tile Scanning when seen on the display
- Correcting fluorescence channel mis-registration when using DIC