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Leica TCS SP5 Confocal Microscope


Location: MRC Building, first floor, room 1.20B

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titleAccess & Usage

Training

Confocal Booking System (Faces Scheduling System)

Microscope Booking Rules

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titleTechnical Specifications
Microscope Information
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titleUser Guides

Confocal Resolution Calculator

Start-up Procedure

Shut-down Procedure

Image Acquisition

Using the Hybrid Detectors

How to insert the sample holder

Image Orientation

Orientation of Stage Movement

Using Tile Scan

Direction of Tile Scanning when seen on the display

Correcting fluorescence channel mis-registration when using DIC

Correcting chromatic shift using Volocity

Making symmetric stacks in Mark & Find mode

Making asymmetric stacks in Mark & Find mode using Define Asymmetry

Introduction to Live Data Mode - Capturing images for SRRF

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The SP5 is a flexible, fully-featured laser scanning confocal microscope suitable for imaging fixed and live samples. It has an inverted DMI6000B microscope stand capable of fluorescence, bright-field and DIC techniques. The SP5 is a tandem scanner system, which means that it has both a conventional scanner and a fast (8000Hz) resonant scanner for high speed imaging. It is fitted with 5 lasers, producing 8 potential excitation lines, and has 4 PMT detectors and one transmitted light detector. Optical sectioning of samples is achieved using the microscope's integrated focus drive. The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas. FRAP, FRET AB (Acceptor Photobleaching), FRET SE (Sensitised Emission) and Live Data Mode modules are also included with the software. A Pe-Con stage-top incubation system with heat and CO2 control is available for live samples.