Leica TCS SP5 Confocal Microscope
Location: MRC Building, first floor, room 1.20B
The SP5 is a flexible, fully-featured laser scanning confocal microscope suitable for imaging fixed and live samples. It has an inverted DMI6000B microscope stand capable of fluorescence, bright-field and DIC techniques. The SP5 is a tandem scanner system, which means that it has both a conventional scanner and a fast (8000Hz) resonant scanner for high speed imaging. It is fitted with 5 lasers, producing 8 potential excitation lines, and has 4 PMT detectors and one transmitted light detector. Optical sectioning of samples is achieved using the microscope's integrated focus drive. The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas. FRAP, FRET AB (Acceptor Photobleaching), FRET SE (Sensitised Emission) and Live Data Mode modules are also included with the software. A Pe-Con stage-top incubation system with heat and CO2 control is available for live samples.
Contents |
Access & Usage
Technical Specifications
User Guides
- Confocal Resolution Calculator
- Start-up Procedure
- Shut-down Procedure
- Image Acquisition
- Using the Hybrid Detectors
- How to insert the sample holder
- Image Orientation
- Orientation of Stage Movement
- Using Tile Scan
- Direction of Tile Scanning when seen on the display
- Correcting fluorescence channel mis-registration when using DIC
- Correcting chromatic shift using Volocity
- Making symmetric stacks in Mark & Find mode
- Making asymmetric stacks in Mark & Find mode using Define Asymmetry
- Introduction to Live Data Mode - Capturing images for SRRF