The Leica TCS SPE and SPE2 are almost identical basic "personal" confocal microscopes mounted on DM2500 upright microscope stands. They are designed principally for scanning samples that have been fixed and mounted on slides but they can also be used to image living samples if they can be mounted using gaskets and imaged through coverslips (e.g. Drosophila pupae). Alternatively we have a set of water dipping lenses for use with samples that require open chambers. Incubation at 37°C can be done using either a Warner Instruments perfusion system or Okolab instrumentation designed for use with dipping lenses on upright microscopes. Please email lmcb-lm-help@ucl.ac.uk well in advance if you want to use CO₂ as a bottle of the right percentage will have to be supplied. Both machines have four lasers and a single detector so all multichannel imaging must be done sequentially.