DM2500 Microscope
The SPE confocals are built around manual DM2500 microscope stands. The fluorescence filter turret and objective lens nosepiece are manually rotated. There is no internal focus motor; confocal sectioning is achieved via a galvanometer driven stage insert. Eyepiece or confocal light path selection is via manual slider. DIC components are available for these microscopes.
Magnification |
Immersion Medium |
Designation |
Numerical Aperture |
Coverslip Correction |
DIC Prism |
|---|---|---|---|---|---|
10x |
Air |
ACS APO |
0.3 |
None |
C |
20x |
Multi |
ACS APO |
0.6 |
0 - 0.17 |
|
40x |
Oil |
ACS APO |
1.15 |
0.17 |
E |
63x |
Oil |
ACS APO |
1.3 |
0.17 |
E |
*63x |
Water Dipping |
HCX APO |
0.9 |
0 |
|
**40x |
Water Dipping |
HCX APO |
0.8 |
0 |
|
**20x |
Water Dipping |
HCX APO |
0.5 |
0 |
|
*Objective only available on the SPE not the SPE2
**Objective only available on the SPE2 not the SPE
Turret Position |
Filter Set |
Excitation Filter |
Dichroic Mirror |
Emission Filter |
Example Fluorophores |
|---|---|---|---|---|---|
1 |
N2.1 |
BP515-560 |
RKP580 |
LP590 |
Texas Red |
2 |
I3 |
BP450-490 |
RKP510 |
LP515 |
FITC/GFP |
3 |
A |
BP340-380 |
RKP400 |
LP425 |
Hoechst/DAPI |
4 |
BF |
N/A |
N/A |
N/A |
Transmitted Light/Confocal |
5 |
*B/G/R |
BP 420/30; 495/15; 570/20 |
415; 510; 590 |
BP 465/20; 530/30; 640/40 |
Blue, Green and Red |
*B/G/R is not currently installed on SPE2