SPE System Information

DM2500 Microscope

The SPE confocals are built around manual DM2500 microscope stands. The fluorescence filter turret and objective lens nosepiece are manually rotated. There is no internal focus motor; confocal sectioning is achieved via a galvanometer driven stage insert. Eyepiece or confocal light path selection is via manual slider. DIC components are available for these microscopes.

Objective lenses on the Leica DM2500 Microscope

Magnification	Immersion Medium	Designation	Numerical Aperture	Coverslip Correction	DIC Prism
10x	Air	ACS APO	0.3	None	C
20x	Multi	ACS APO	0.6	0 - 0.17
40x	Oil	ACS APO	1.15	0.17	E
63x	Oil	ACS APO	1.3	0.17	E
*63x	Water Dipping	HCX APO	0.9	0
**40x	Water Dipping	HCX APO	0.8	0
**20x	Water Dipping	HCX APO	0.5	0

*Objective only available on the SPE not the SPE2
**Objective only available on the SPE2 not the SPE

Fluorescence filter sets on the Leica DM2500 microscope

Turret Position	Filter Set	Excitation Filter	Dichroic Mirror	Emission Filter	Example Fluorophores
1	N2.1	BP515-560	RKP580	LP590	Texas Red
2	I3	BP450-490	RKP510	LP515	FITC/GFP
3	A	BP340-380	RKP400	LP425	Hoechst/DAPI
4	BF	N/A	N/A	N/A	Transmitted Light/Confocal
5	*B/G/R	BP 420/30; 495/15; 570/20	415; 510; 590	BP 465/20; 530/30; 640/40	Blue, Green and Red

*B/G/R is not currently installed on SPE2