Fluorescence Filters

Owned by Ki Hng
Sept 05, 2017

Fluorescence filter sets on the Leica DM2500 microscope

Turret Position

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Example Fluorophores

1

N2.1

BP515-560

RKP580

LP590

Texas Red

2

I3

BP450-490

RKP510

LP515

FITC/GFP

3

A

BP340-380

RKP400

LP425

Hoechst/DAPI

4

BF

N/A

N/A

N/A

Transmitted Light/Confocal

5

*B/G/R

BP 420/30; 495/15; 570/20

415; 510; 590

BP 465/20; 530/30; 640/40

Blue, Green and Red

*B/G/R is not currently installed on SPE2