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Fluorescence Filters

Fluorescence Filters

Owned by Ki Hng
Sept 05, 2017

Fluorescence filter sets on the Leica DM2500 microscope

Turret Position

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Example Fluorophores

1

N2.1

BP515-560

RKP580

LP590

Texas Red

2

I3

BP450-490

RKP510

LP515

FITC/GFP

3

A

BP340-380

RKP400

LP425

Hoechst/DAPI

4

BF

N/A

N/A

N/A

Transmitted Light/Confocal

5

*B/G/R

BP 420/30; 495/15; 570/20

415; 510; 590

BP 465/20; 530/30; 640/40

Blue, Green and Red

*B/G/R is not currently installed on SPE2

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